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Effects of resuscitation with three kinds of fluid on liver oxygen free radical in rats with severe hemorrhagic shock

Author(s): Hua-mei LU | Li-juan WEI | Mu-qiang YANG | Zhi-long GENG | Feng ZHAO

Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402

Volume: 37;
Issue: 12;
Start page: 1111;
Date: 2012;
Original page

Keywords: shock | hemorrhagic | resuscitation | reactive oxygen species

Objective  To explore effects of resuscitation with different kinds of fluid on liver oxygen free radical in rats with severe hemorrhagic shock. Methods  Sixty SD Rats were randomly divided into 5 groups (n=12): control group (C), shock alone group (SA), lactated Ringer's solution (LR)-treatment group, hydroxyethyl starch (HES)-treatment group, 7.2% hypertonic saline-6% HES (HSH)-treated group. According to Wigger's method, the rat model of severe hemorrhagic shock was reproduced. The mean arterial pressure (MAP) was monitored in all the 5 groups. The ALT and AST level in venous blood were determined before shock (Tb), 1h after shock (Ts) and 4h after resuscitation (Td) respectively. In SA group, the rats were sacrificed and the livers were collected at Ts. The rats were resuscitated with LR (3 times the volume of shed blood), HES (1 time the volume of shed blood) and HSH (6ml/kg) in LR, HES and HSH group respectively. The rats in groups C, LR, HES and HSH were sacrificed at Td to obtain the liver tissues. The expression of TNF-α, NF-κB protein, MDA and SOD activities were determined by immunohistochemistry (SP) and chromatometry. The iNOS content was detected by ELISA. The livers were also observed under light microscope and electron microscope for pathological changes. Results  Compared with group C, the AST and ALT levels in other groups were significantly higher, the expressions of NF-κB, iNOS and MDA in liver significantly increased, while SOD activity significantly decreased (P<0.05). The signs of injury were remarkable. The content of cytokines was significantly increased in blood in 3 fluid resuscitation groups compared with group SA (P<0.05), and signs of tissue injury were also significant. In 3 fluid resuscitation groups, the increase in cytokines was most remarkable in group LR (P<0.05), and liver tissue injury was also most remarkable, while the cytokine levels were lower (P<0.05) and pathological changes were also milder in HSH group. Conclusions  HSH resuscitation produces loss liver injury after severe hemorrhagic shock by decreasing cytokine production and reducing oxygen free radical-mediated lipid peroxidation.

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