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Effects of resveratrol on matrix metalloproteinase-9 expression in hepatoma cells

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Author(s): Hai-bo YU

Journal: Zhong Xi Yi Jie He Xue Bao
ISSN 1672-1977

Volume: 6;
Issue: 3;
Start page: 270;
Date: 2008;
Original page

Keywords: resveratrol | liver cancer | matrix metalloproteinase-9

ABSTRACT
Objective: To observe the effects of resveratrol on proliferation of human hepatocellular carcinoma cell line SMMC-7721 cells and expression of matrix metalloproteinase-9 (MMP-9) in vitro.Methods: SMMC-7721 cells were treated with different concentrations of resveratrol for 24, 48 and 72 h, respectively. The effect of resveratrol on proliferation of SMMC-7721 cells was assessed with methyl thiazolyl tetrazolium (MTT). The expression of MMP-9 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR). MMP-9 protein was identified by Western blot analysis.Results: Resveratrol could inhibit the proliferation of SMMC-7721 cells with dose- and time-dependent effects. Moreover, both MMP-9 mRNA expression and MMP-9 protein production were markedly reduced after resveratrol treatment. Conclusion: Resveratrol can inhibit the proliferation of SMMC-7721 cells and down-regulate MMP-9 expression. It is presumed that resveratrol may suppress the invasion and metastasis of hepatocellular carcinoma.

Tango Jona
Tangokurs Rapperswil-Jona

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