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Effects of Two Different Levels of Dietary Protein on Body Composition and Protein Nutritional Status of Growing Rats

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Author(s): Julio Tirapegui | Sandra Maria Lima Ribeiro | Ivanir Santana de Oliveira Pires | Marcelo Macedo Rogero

Journal: Nutrients
ISSN 2072-6643

Volume: 4;
Issue: 9;
Start page: 1328;
Date: 2012;
Original page

Keywords: dietary protein | protein nutritional status | growth | body composition

ABSTRACT
This study aimed to investigate the effect of a high-protein diet on growth, body composition, and protein nutritional status of young rats. Newly-weaned Wistar rats, weighing 45–50 g, were distributed in two experimental groups, according to their diets, which contained 12% (G12) or 26% protein (G26), over a period of 3 weeks. The animals were euthanized at the end of this period and the following analyses were performed: chemical composition of the carcass, proteoglycan synthesis, IGF-I concentration (serum, muscle and cartilage), total tissue RNA, protein concentration (muscle and cartilage) and protein synthesis (muscle and cartilage). The high-protein diet was found to result in a higher fat-free mass and lower fat mass in the carcass, with no difference in growth or protein nutritional status.

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