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Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK

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Author(s): Luo Jie | Sun Xin | Gao Feng | Zhao Xiaoliang | Zhong Biao | Wang Hong | Sun Zhijun

Journal: Journal of Experimental & Clinical Cancer Research
ISSN 1756-9966

Volume: 30;
Issue: 1;
Start page: 71;
Date: 2011;
Original page

ABSTRACT
Abstract Objective To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. Methods The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. Their effects on 1) cell invasion ability was assayed using Transwell; 2) expression of uPA, uPAR and ERK was detected by real time PCR and Western blot; 3) uPA, uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry. Results 1) Treatment with ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively, significantly inhibited MDA-MB-231 and MCF-7 cell invasion; 2) mRNA and protein levels of uPA, uPAR and ERK1/2 were inhibited by ulinastatin, but enhanced by docetaxel. Conclusion Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA, uPAR and p-ERK expression is obviously inhibited by ulinastatin.
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