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Efficacies of -L-D4A against hepatitis B virus in 2.2.15 cells

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Author(s): Lin-Lin Gao, Xiao-Yan Wang, Ju-Sheng Lin, Ying-Hui Zhang, Yan Li

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 14;
Issue: 8;
Start page: 1263;
Date: 2008;
Original page

Keywords: 2’ | 3’-didehydro-2’ | 3’-dideoxyadenosine | 2.2.15 cells | Hepatitis B virus DNA | Hepatitis B surface antigen

ABSTRACT
AIM: To investigate the antiviral effect of beta-L-enantiomer of 2’,3’-didehydro-2’,3’-dideoxyadenosine (β-L-D4A) on 2.2.15 cells transfected with the hepatitis B virus (HBV) genome.METHODS: Lamivudine (3TC) as a positive control. Then, HBV DNA in treated 2.2.15 cells and the Hepatitis B surface antigen (HBsAg) in the culture supernatants were detected to determine the inhibitory effect of β-L-D4A. At the same time, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to detect the survival ratio of 2.2.15 cells.RESULTS: β-L-D4A has a dose-dependent inhibitory effect on HBV DNA replication; this effect was apparent when the concentration was above 1 mol/L. When β-L-D4A was at the highest concentration, 100 mol/L, the HBsAg inhibition ratio was above 50%. The Therapeutic index (TI) of β-L-D4A was above 2.1.CONCLUSION: β-L-D4A has a dose-dependent inhibitory effect on the replication of HBV DNA and the secretion of HBsAg at low toxicity.
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