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The efficacy of milk ejection induced by luteal oxytocin as a method of early pregnancy diagnostics in cows

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Author(s): Grizelj J. | Katana B. | Dobranić T. | Prvanović Nikica | Lipar Marija | Vince S. | Stanin D. | Đuričić D. | Gregurić-Gračner Gordana | Samardžija M.

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 60;
Issue: 5-6;
Start page: 551;
Date: 2010;
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Keywords: cow | luteal oxytocin | milk progesterone | pregnancy diagnosis

ABSTRACT
The aim of this research was to determine the accuracy and reliability of early diagnosis of pregnancy in cows using PGF2α analogue. Namely in the case of corpus luteum (CL) presence, the release of luteal oxytocin is induced by intravenous administration of a non luteolytic dose of PGF2α. The research was performed in 30 lactating cows, 18-22 days after insemination, and three hours prior to evening milking. A teat cannula is placed in the left fore-teat. When the cisternal milk flow ceased, Dinoprost (256 μg Dinolytic) was injected in the v. jugularis. If CL was present, the alveolar milk flow (559.4±83.7 mL) started 189±18 s later and early pregnancy could be presumed. If CL was no longer functional, the milk flow did not start again and the cow was considered not pregnant. Seventeen out of 30 cows were confirmed pregnant 35 days after insemination by ultrasound and 13 were found not pregnant. The accuracy of positive results is 100% and is identical to that obtained by ELI
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