Academic Journals Database
Disseminating quality controlled scientific knowledge

Efflux Pumps Modulation in Colorectal Adenocarcinoma Cell Lines: The Role of Nuclear Medicine

Author(s): João Casalta-Lopes | Ana Margarida Abrantes | Mafalda Laranjo | Joana Rio | Ana Cristina Gonçalves | Bárbara Oliveiros | Ana Bela Sarmento-Ribeiro | Maria Filomena Botelho

Journal: Journal of Cancer Therapy
ISSN 2151-1934

Volume: 02;
Issue: 03;
Start page: 408;
Date: 2011;
Original page

Keywords: Multidrug Resistance | P-Glycoprotein | Multidrug Resistance-Associated Protein 1 | Major Vault Protein

Introduction: Multidrug resistance (MDR) is one of the major problems of chemotherapy. Overexpression of efflux pumps, such as P-glycoprotein (Pgp), multiple resistance-related protein 1 (MRP-1) and lung resistance protein (LRP) can lead to MDR. Verapamil and L-buthionine-sulfoximine (BSO) are two modulators of these proteins. This study aims to compare 99mTc-Sestamibi transport kinetics in human colorectal adenocarcinoma cell lines, in the presence and absence of the MDR modulators verapamil and BSO. Material and Methods: MDR proteins expression was evaluated in sensitive (WiDr) and resistant (LS1034) human colorectal adenocarcinoma cell lines. Intracellular and plasma membrane Pgp and MRP1, and LRP expression was analyzed by flow-cytometry and western blot. Cellular transport kinetics was assessed using 99mTc-Sestamibi. MDR modulation was evaluated though retention studies in resistant cells after incubation with the modulators. Results: Pgp expression was significantly higher (p≤0.001) in resistant cells. These results were confirmed by western blot analysis. 99mTc-Sestamibi uptake and retention percentage were significantly higher (p
Why do you need a reservation system?      Save time & money - Smart Internet Solutions