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<em>luxS</em> Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?

Author(s): Qian Wang | Zhiyan He | Yuejian Hu | Yuntao Jiang | Rui Ma | Zisheng Tang | Jingping Liang | Zheng Liu | Zhengwei Huang

Journal: Sensors
ISSN 1424-8220

Volume: 12;
Issue: 5;
Start page: 6176;
Date: 2012;
Original page

Keywords: quorum sensing | AI-2 | luxS | sahH

AI-2–mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system—not the metabolic aspect—is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation.
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