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An Endogenous Immune Adjuvant Released by Necrotic Cells for Enhancement of DNA Vaccine Potency

Author(s): Rohollah Dorostkar | Taravat Bamdad | Masoud Parsania | Hassan Pouriayevali

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 9;
Issue: 4;
Start page: 215;
Date: 2012;
Original page

Keywords: Adjuvant | DNA Vaccine | Herpes Simplex Virus-1 | Necrosis

Background: Improving vaccine potency in the induction of a strong cell-mediated cytotoxicity can enhance the efficacy of vaccines. Necrotic cells and the supernatant of necrotic tumor cells are attractive adjuvants, on account of their ability to recruit antigen-presenting cells to the site of antigen synthesis as well as its ability to stimulate the maturation of dendritic cells. Objective: To evaluate the utility of supernatant of necrotic tumor cells as a DNA vaccine adjuvant in a murine model. Method: The supernatant of EL4 necrotic cells was co-administered with a DNA vaccine expressing the glycoprotein B of Herpes simplex virus-1 as an antigen model under the control of Cytomegalovirus promoter. C57BL/6 mice were vaccinated three times at two weeks intervals with glycoprotein B DNA vaccine and supernatant of necrotic EL4 cells. Five days after the last immunization, cell cytotoxicity, IFN-γ and IL-4 were evaluated. Results: The obtained data showed that the production of IFN-γ from the splenocytes after antigenic stimulation in the presence of the supernatant of necrotic EL4 cells was significantly higher than the other groups (p

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