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Enzymatic Digestion of Single DNA Molecules Anchored on Nanogold-Modified Surfaces

Author(s): Lü Junhong | Ye Ming | Duan Na | Li Bin

Journal: Nanoscale Research Letters
ISSN 1931-7573

Volume: 4;
Issue: 9;
Start page: 1029;
Date: 2009;
Original page

Keywords: Gold nanoparticles | Mica | DNA | Atomic force microscopy | Dip-Pen Nanolithography

Abstract To study enzyme–DNA interactions at single molecular level, both the attachment points and the immediate surroundings of surfaces must be carefully considered such that they do not compromise the structural information and biological properties of the sample under investigation. The present work demonstrates the feasibility of enzymatic digestion of single DNA molecules attached to nanoparticle-modified surfaces. With Nanogold linking DNA to the mica surface by electrostatic interactions, advantageous conditions with fewer effects on the length and topography of DNA are obtained, and an appropriate environment for the activities of DNA is created. We demonstrate that by using Dip-Pen Nanolithography, individual DNA molecules attached to modified mica surfaces can be efficiently digested by DNase I.
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Tango Jona
Tangokurs Rapperswil-Jona