Academic Journals Database
Disseminating quality controlled scientific knowledge

Establishment of an Indirect Genetic Transformation Method for Arabidopsis thaliana ecotype Bangladesh

ADD TO MY LIST
 
Author(s): Bulbul AHMED | Mansura KHATUN | Monzur HOSSAIN | Rafiul ISLAM | Manosh BISWAS | Abdul MANDAL

Journal: Journal of Central European Agriculture
ISSN 1332-9049

Volume: 12;
Issue: 3;
Start page: 519;
Date: 2011;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: agrobacterium tumefaciens | arabidopsis thaliana | brassicaceae | bangladesh

ABSTRACT
Arabidopsis thaliana is a small flowering plant belonging to the Brassicaceae family, which is adopted as a model plant for genetic research. Agrobacterium tumifaciensmediated transformation method for A. thaliana ecotype Bangladesh was established. Leaf discs of A. thaliana were incubated with A. tumefaciens strain LBA4404 containing chimeric nos. nptII. nos and intron-GUS genes. Following inoculation and co-cultivation, leaf discs were cultured on selection medium containing 50 mg/l kanamycin + 50 mg/l cefotaxime + 1.5 mg/l NAA and kanamycin resistant shoots were induced from the leaf discs after two weeks. Shoot regeneration was achieved after transferring the tissues onto fresh medium of the same combination. Finally, the shoots were rooted on MS medium containing 50 mg/l kanamycin. Incorporation and expression of the transgenes were confirmed by PCR analysis. Using this protocol, transgenic A. thaliana plants can be obtained and indicates that genomic transformation in higher plants is possible through insertion of desired gene. Although Agrobacterium mediated genetic transformation is established for A. thaliana, this study was the conducted to transform A. thaliana ecotype Bangladesh.
Save time & money - Smart Internet Solutions      Why do you need a reservation system?