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Establishment and Molecular Cytogenetic Characterization of a Cell Culture Model of Head and Neck Squamous Cell Carcinoma (HNSCC)

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Author(s): Verena L. Bauer | Ludwig Hieber | Quirin Schaeffner | Johannes Weber | Herbert Braselmann | Reinhard Huber | Axel Walch | Horst Zitzelsberger

Journal: Genes
ISSN 2073-4425

Volume: 1;
Issue: 3;
Start page: 388;
Date: 2010;
Original page

Keywords: HNSCC | CAL 33 | cell lines | cytogenetics

ABSTRACT
Cytogenetic analysis of head and neck squamous cell carcinoma (HNSCC) established several biomarkers that have been correlated to clinical parameters during the past years. Adequate cell culture model systems are required for functional studies investigating those potential prognostic markers in HNSCC. We have used a cell line, CALĀ 33, for the establishment of a cell culture model in order to perform functional analyses of interesting candidate genes and proteins. The cell line was cytogenetically characterized using array CGH, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH). As a starting point for the investigation of genetic markers predicting radiosensitivity in tumor cells, irradiation experiments were carried out and radiation responses of CAL 33 have been determined. Radiosensitivity of CAL 33 cells was intermediate when compared to published data on tumor cell lines.
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