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Estudio del efecto inhibitorio de extractos de Salvia scutellarioides sobre la actividad de la enzima convertidora de angiotensina

Author(s): Raúl Arenas-Carvajal | Erica Pachón-Gómez | Gina Méndez-Callejas | Antonio Guzmán-Avendaño

Journal: Universitas Scientiarum
ISSN 0122-7483

Volume: 14;
Issue: 2-3;
Start page: 141;
Date: 2009;
Original page

Keywords: angiotensin II | angiotensin-converting enzyme | arterial hypertension | enzymatic activity | Salvia scutellarioides

Study of the inhibitory effect of Salvia scutellarioides extracts on the activity of the angiotensin-converting enzyme. Objective. Toidentify groups of secondary metabolites of Salvia scutellarioides present in the fraction with the greatest inhibitory effect on the activity ofthe angiotensin-converting enzyme (ACE). Materials and methods. Dry plant material was used to prepare ethanolic extracts that werethen concentrated and separated by column chromatography using solvents of different polarity (petroleum ether, dichloromethane, diethylether and ethanol). Subsequently, lung tissue isolated from Wistar rats was broken and centrifuged in order to separate the soluble material.Proteins found in the supernatant were separated using a Sephacryl column; a pool was made with the fractions that showed activity withhippuryl-L-histidyl-L-leucine (HHL), the substrate of the ACE. This enzyme extract was used to measure the effect of plant extractsobtained from S. scutellarioides on the ACE activity. Results: The fraction of ethyl acetate (T2) showed a greater inhibitory effect on theACE activity. Metabolites found in T2 fraction were: tannins, cardiac glycosides, coumarins, and quinones. Conclusion. An antihypertensiveeffect was found for the plant species Salvia scutellarioides by studying the inhibitory effect on the ACE activity. Several groups ofsecondary metabolites present in the T2 fraction were identified, which could be responsible for this effect.
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