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EVA GREEN REAL-TIME PCR USED TO DETECT CELERY AS AN ALLERGEN IN FOOD

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Author(s): Ondrej Škultéty | Andrea Jurčáková

Journal: Potravinarstvo : Scientific Journal for Food Industry
ISSN 1338-0230

Volume: 5;
Issue: 2;
Start page: 70;
Date: 2011;
Original page

Keywords: apium graveolens | food analysis | detection limit | polymerase chain reaction

ABSTRACT
EvaGreen®  Real-Time PCR method has been used for celery(Apium graveolens) allergen detection. A primer designed in mannitol dehydrogenase gene region has been used for specific celery identification in sample. The results show possibility to create calibration curve using artificially adulterated samples. The increasing variability between parallel calibration of celery samples has been observed from 0.1 % to 100%. Detection limit has been set to value 0.1% in celery representing 1000 ppm. Fluorescent signal has been presented even in samples with lower percentage addition of celery but these samples have been excluded according to unspecific melting curve.doi:10.5219/138

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