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Evaluating microalgae Chlorella sorokiniana growth in different culture mediums in autotrophic and mixotrophic conditions

Author(s): Martha L. Ortiz- Moreno | Caroll E. Cortés - Castillo | Julián Sánchez-Villarraga | Jorge Padilla | Angélica M. Otero-Paternina

Journal: Orinoquia
ISSN 0121-3709

Volume: 16;
Issue: 1;
Start page: 11;
Date: 2012;
Original page

Keywords: Chlorella sorokiniana | cell density | poultry manure leachate | mixotrophy | autotrophy

AbstractChlorella sorokiniana is a microalgae having great biotechnological potential due to its ability to synthesise fatty acids having industrial interest, rapid growth and the ability to adapt to differing nutrient sources in both autotrophic and mixotrophic regimens, and even heterotrophic ones. This work was aimed at identifying the culture medium which would lead to maximum C. sorokiniana growth to enable its mass production. Sueoka, Guillard and Remital culture mediums (1, 2,3, 4, 5g/L NPK fertiliser) were evaluated; they offer nutrients in autotrophic conditions and 30%, 50% and 80% concentration poultry manure leachate in a mixotrophic regimen. 86.5±0.75 x 107 cells/ml maximum cell density and mean 0.3 generations/day specific growth rate were recorded in Remital medium. Tukey and Fisher statistical tests indicated that Remital was the best culture medium and that autotrophic conditions were ideal for C. sorokiniana growth. Using high concentrations of Remital did not significantly improve microalgae growth. Using low concentrations of Remital (1g/L) is thus recommended as the best option for mass production of C. sorokiniana biomass.
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