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Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples

Author(s): Thais Moura Gascón | Fernanda Schindler | Claudia Giorgia Bronzatti de Oliveira | Fabiola Isabel Suano Souza | Sonia Hix | Roseli Oselka Sacardo Sarni | Ana Paula Arantes Bacan | Vania D’Almeida | Auro del Giglio | Fernando Luiz Affonso Fonseca

Journal: Einstein
ISSN 1679-4508

Volume: 8;
Issue: 2;
Start page: 187;
Date: 2010;
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Keywords: Homocysteine | Chromatography | high pressure liquid | Chemiluminescences measurements

Objective: To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. Methods: A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. Results: The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 μmol/l (r = 0.821 and p < 0.001). Conclusions: It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases.

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