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Evaluation of Chronic Hepatitis B Infection in Patients With Seronegative HbsAg

Author(s): E Gholami Parizad | A Khosravi | E Gholami Parizad | N Sadeghifard | S Ghafourian

Journal: Iranian Journal of Public Health
ISSN 2251-6085

Volume: 41;
Issue: 2;
Start page: 100;
Date: 2012;
Original page

Keywords: Hepatitis B | HBsAg | Serum | Carrier | Ear Cerumen

Background: It is estimated that about 370 million people are chronic carriers of HBV worldwide. Apparently 3% of Iranian populations are chronic carriers of this virus. We aimed to evaluate the viral DNA in biological fluids of chronic hepatitis patients compared to a control group.Methods: The current case-control study was designed to evaluate the viral DNA in biological fluids of 70 chronic hepatitis patients compared to a control group using ELISA, PCR and Real Time.Results: All individuals (100%) in case group were HBsAg positive while in control group only 2 individuals (2.8%) were HBsAg positive. Three individuals , in control group were positive using PCR and Real Time PCR indicating that about 7% of those in control group were chronic carriers of HBV. The interesting point was the copy of viral DNA; (5.49 ×104, 2.162×103and 7.26×106) for 3 chronic carriers using sera while it was about (5.71×103, 1.45×102 and 2.56×105) using ear cerumen confirming the necessity of investigating for the carriers of HBV in different biological fluid and by different methods.Conclusion: It can be concluded chronic carriers of hepatitis B are much more than what is diagnosed by routine diagnostic tests. On the other hand ELISA alone can not be relied on as a complete test for screening of chronic carriers in hepatitis B. PCR and Real Time PCR are more reliable tests for this purpose.
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