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Evaluation of different plating medias and PCR in the detecting of Salmonella Enteritidis from eggs laid by experimentally infected hens

Author(s): M. S. Madadi1 | M. Azari | A. Yazdani

Journal: Journal of Animal and Poultry Sciences
ISSN 2147-9267

Volume: 1;
Issue: 1;
Start page: 13;
Date: 2012;
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Keywords: Salmonella Enteritidis | PCR | Culture | hen

Salmonellosis is one of the most important food-borne diseases. Outbreaks of Salmonellosis are mainly related to the consumption of contaminated eggs or egg-products and, less frequently, of poultry meat. In this study fifty 29-week-old broiler breeder hens were randomly divided in two groups of 25 birds. One group of birds inoculated orally with 10log10 CFU and other group inoculated intravenously with 6log10 CFU of S. Enteritidis parent strain bacteria. During 35 days of experimental period, produced eggs were cultured using different medias and S.Enteritidis was detected using different cultural and PCR method. SE was isolated during 32.5 % of experimental days and more isolation rate of Salmonella was during the first two weeks of post infection period. In contrast, using pre-enrichment media resulted more isolation rate of Salmonella cells and SS agar was more sensitive than BG agar. PCR was more sensitive than cultural methods for detection of Salmonella Enteritidis from contaminated eggs.
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