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EVALUATION OF ELISA METHOD TO DETECTION OF COW β-LACTOGLOBULIN IN SHEEP MILK AND SHEEP MILK PRODUCTS

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Author(s): Lucia Zeleňáková | Radoslav Židek | Margita Čanigová | Juraj Paulov | Tatiana Gallisová

Journal: Potravinarstvo : Scientific Journal for Food Industry
ISSN 1338-0230

Volume: 4;
Issue: 4;
Start page: 80;
Date: 2010;
Original page

Keywords: elisa | milk | cheese | Slovak sheep cheese

ABSTRACT
The aim of work was to optimalize the ELISA method to detect the adulteration of sheep milk and sheep milk products by cow milk in the laboratory. We have focused on laboratory testing of ELISA kit (β-Lactoglobulin ELISA Set, SEDIUM R&D) for detection of cow β-Lg in sheep milk order to obtain high-quality, reliable and economically advantageous method suitable for routine use in practice. The results shown that for the quality of adulteration determination  it is necessary to verify the sensitivity of applied kit by the samples dilution in accordance with the producer declared quantification range contained in the manual ELISA kit. The starting point for obtaining of relevant data was to create separate regression curves with high deter­mination coefficient, which allowed to quickly and easily detect the cow milk additions in sheep milk, cloddish sheep and Slovak sheep cheese. doi:10.5219/78  
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