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Evaluation of five protocols of conservation‘s techniques in chiguiro Hydrochaeris hydrochaeris (Linnaeus, 1766) tissues

Author(s): José R. Corredor-Matus | Carlos J. Sejín-Puche | Mario A. González- G

Journal: Orinoquia
ISSN 0121-3709

Volume: 14;
Issue: S1;
Start page: 110;
Date: 2010;
Original page

Bodies conservation is a technique that uses the formalin chemical almost exclusively. It´s use dehydrates the tissues, takes the tissues dissection very hard and volatilized contaminants fumes. With the purpose to carry out morphological studies in chigüiros (Hydrochaeris hydrochaeris) and minimize contaminants effects for exclusive use of formalin, tests were made with five conservations protocol´s, using compounds such: Formalin at 5, 12, 14, 16 y 18 %; Phenol at 3, 5, 7 and 8 %; glycerin at 1, 5 and 10 %; Ethanol at 5, 7, 10 y 12 %, using two experimental animals for each one. Specimens were anesthetized with a mixture of atropine, midazolam, ketamine and xylazine. Exsanguinations and application of conservation protocols, was made through the carotid artery, using an injection fluids pump. Volume applied was calculated according with ten per cent of corporal weight of each specimen. Dissection was begun ten days after conserving protocols applied. Muscle tissues were took to different times (30, 60, 90, 120 after protocols applications), to verified the actions of each protocol. Protocol 1 is behaving properly, because the corpses were preserved in good condition during the study period, allowing the realization of dissections and supported the manipulation of parts, protecting them from the climatic conditions prevailing in the area. The protocol two and three had similar behavior to one, the tissues were softer, but more dehydrated by the use of higher concentrations of formaldehyde. It is noteworthy that vapor contamination wassignificantly reduced. In protocol 4, the tissues of the head, neck, upper extremities and thoracic viscera, were kept well hydrated and better. Protocol 5, follows the same pattern of others. The preservation of tissues was good, with no perceived excess volatility of gas, the dissection carried out more comfortably.Still need to analyze the histological studies relating to the last sampling (120 days) to determine the effect on the tissues. It is expected; the study is completed, and has protocols that ensure greater conservation, soft tissue and substantial decrease in gas pollution.
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