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Evaluation of Hepatic Microsomal Enzyme Functional Integrity on Picroliv Pretreatment Against CCl4 Induced Hepatotoxicity

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Author(s): P. Bigoniya | A. Shukla | C.S. Singh

Journal: International Journal of Pharmacology
ISSN 1811-7775

Volume: 6;
Issue: 3;
Start page: 200;
Date: 2010;
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Keywords: carbon tetrachloride | hepatoprotective | Picroliv | cytochrome P450 | bromosulphalein

ABSTRACT
The effect of picroliv isolated from Picrorrhiza kurroa was evaluated at different dose ranges to establish its ED50 against carbon tetrachloride (CCl4) induced hepatotoxicity. The CCl4 was given orally (1 mL kg-1 b.wt.) in liquid paraffin (1:1). Picroliv at different doses and silymarin (20 mg kg-1 b.wt.) standard were administered orally for 14 days. Body weight, biochemical parameters like Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT) and Alkaline Phosphatase (ALP) were estimated. Hepatic microsomal drug metabolizing enzyme functional integrity was accessed by in vitro bromosulphalein (BSP) uptake test of liver slices and thiopental (40 mg kg-1 b.wt.) induced sleeping time. The results showed dose related hepatoprotective efficacy of picroliv. The ED50 values for effectiveness of liver enzyme metabolizing activity on BSP uptake test and sleeping time were 11.94 and 17.49 mg kg-1 b.wt., respectively.
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