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Evaluation of larvicidal activity of soil microbial isolates (Bacillus and Acinetobactor Sp.) against Aedes aegypti (Diptera: Culicidae) - the vector of Chikungunya and Dengue

Author(s): D. Radhika | A. Ramathilaga | C. Sathesh Prabu, et al.

Journal: Proceedings of the International Academy of Ecology and Environmental Sciences
ISSN 2220-8860

Volume: 1;
Issue: 3-4;
Start page: 169;
Date: 2011;
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Keywords: Aedes aegypti | Bacillus thuringiensis | Bacillus sphaericus | lethal concentration

To isolate and identify the natural non- harmful microbial population from the soil sample for the control of many epidemiological disease causing vector of Aedes aegypti mosquito larvae via larvicidal activity. Heat treat and non heat treated soil samples were used for the isolation of spore forming and non- spore forming microbial isolates through spread plate technique. Preliminary and quantitative larvicidal activity was performed against the mosquito larvae. Protein analyses of best microbial isolates were carried out by SDSPAGE technique. For the screening assay ten microbial isolates were used and five were active against chosen mosquito larvae which were identified as B. megaterium, B. sphaericus, B. cereus, B. subtillis and Acinetobactor sp. under laboratory conditions. The two isolates (B. megaterium and Acinetobactor sp.) were considered as most toxic strains followed by B. sphaericus, B. cereus, and B. subtillis with LC90 values 4.1 +-0.39, 2.8 +- 0.17, 3.6 +- 0.37, 2.5 +-0.71, 3.6 +- 0.71 mg/ml respectively under laboratory conditions and ensuring mortality rate was 97% at 48 hrs exposure tests. This study concludes that non spore formers of common microbial isolates from the natural environment were also able to kill the larvae of A.aegypti through their secondary metabolites which are non - toxic to human population.

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