Author(s): Khushboo J Nagdev | Poonam S Deshpande | Rajpal S Kashyap | Hemant J Purohit | Girdhar M Taori | H F Daginawala
Journal: International Journal of Integrative Biology
ISSN 0973-8363
Volume: 4;
Issue: 1;
Start page: 28;
Date: 2008;
Original page
Keywords: Nested PCR | Pleural tuberculosis
ABSTRACT
Diagnosis of Pleural Tuberculosis (PTB) remains as a major challenge till today. Since conventional methods of diagnosis such as culture and smear are ineffective because of paucibacillary nature of these fluids, efficiency of alternative methods such as PCR needs to be evaluated. We have evaluated polymerase chain reaction (PCR) using IS6110 sequence of Mycobacterium tuberculosis, in a nested PCR approach and correlated its efficiency with adenosine deaminase (ADA) activity, and laboratory parameters in the diagnosis of PTB. Our results show that the nested PCR assay with an overall sensitivity and specificity of 68.6% and 66% and with 72.2% sensitivity and 85.7% specificity for patients with complete follow-up can be valuable in the rapid diagnosis of tuberculous pleurisy where conventional methods fail to do so. The combined analysis of nested PCR with biochemical and laboratory investigation can a very useful in evaluating the efficacy of nested PCR as well as can be a good diagnostic approach to achieve a more rapid and precise diagnosis in the cases of PTB
Journal: International Journal of Integrative Biology
ISSN 0973-8363
Volume: 4;
Issue: 1;
Start page: 28;
Date: 2008;
Original page
Keywords: Nested PCR | Pleural tuberculosis
ABSTRACT
Diagnosis of Pleural Tuberculosis (PTB) remains as a major challenge till today. Since conventional methods of diagnosis such as culture and smear are ineffective because of paucibacillary nature of these fluids, efficiency of alternative methods such as PCR needs to be evaluated. We have evaluated polymerase chain reaction (PCR) using IS6110 sequence of Mycobacterium tuberculosis, in a nested PCR approach and correlated its efficiency with adenosine deaminase (ADA) activity, and laboratory parameters in the diagnosis of PTB. Our results show that the nested PCR assay with an overall sensitivity and specificity of 68.6% and 66% and with 72.2% sensitivity and 85.7% specificity for patients with complete follow-up can be valuable in the rapid diagnosis of tuberculous pleurisy where conventional methods fail to do so. The combined analysis of nested PCR with biochemical and laboratory investigation can a very useful in evaluating the efficacy of nested PCR as well as can be a good diagnostic approach to achieve a more rapid and precise diagnosis in the cases of PTB
