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Evaluation of Relation between IL-4 and IFN- Polymorphisms and Type 2 Diabetes

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Author(s): Mohammad Kazemi Arababadi | Ali Akbar Pourfathollah | Saeed Daneshmandi | Gholamhossein Hassanshahi | Ebrahim Rezazadeh Zrandi | Majid Asiabanha Rezaei | Somayeh Eigder | Ali Shamsizadeh

Journal: Iranian Journal of Basic Medical Sciences
ISSN 2008-3866

Volume: 12;
Issue: 42;
Date: 2009;
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Keywords: Interferon-gamma | Interleukin-4 | Polymorphism | Type 2 diabetes

ABSTRACT
Objective(s)Although, type 2 diabetes is the most frequent type of diabetes, its main cause is yet to be clarified. Severalenvironmental and genetic parameters are believed to be involved in diabetes. It has also been establishedthat cytokines play key roles in pathogenesis of diabetes. Expression of cytokines is different from person toperson and in different societies. Several studies showed that polymorphisms of +874 of interferon-gamma(IFN-γ) and -590 of interleukin-4 (IL-4) are associated with the regulation of expression of these genes. Thisstudy was aimed to find polymorphisms of these regions in type 2 diabetes patients.Materials and MethodsIn this experimental study peripheral blood samples were collected from 160 type 2 diabetic patients and 160healthy controls. DNA was extracted by salting out method. Polymorphisms of +874 of IFN-γ and -590 ofIL-4 were analyzed by ARMS-PCR and RFLP-PCR.ResultsOur findings indicated that TT genotype of IFN-γ was increased in type 2 diabetic patients compared to thecontrol but difference was not significant. Our results didn’t show any significant difference between IL-4genotype in diabetic and healthy controls either.ConclusionOur results suggested that TT genotype of IFN-γ can be associated with diabetes. This association can bedescribed by the fact that over expression of IFN-γ shifts immune system to Th1; therefore, pancreatic cellscan be miscarried by immune cells.Keywords: Interferon-gamma, Interleukin-4, Polymorphism, Type 2 diabetes
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