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Evaluation of Six Methods for Extraction of Total RNA from Loquat

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Author(s): Zhang LING | Zhang ZHIKE | Lin SHUNQUAN | Zheng TINGTING | Yang XIANGHUI

Journal: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
ISSN 0255-965X

Volume: 41;
Issue: 1;
Start page: 313;
Date: 2013;
Original page

ABSTRACT
  Trizol extraction for polysaccharide-rich plant tissue was unsuitable for isolating total RNA from loquat fruit. CTAB-LiCl extraction was improved with pretreatment using washing buffer with 80% ethanol and 70% acetone. The RNA isolated by this protocol from different loquat fruit tissue at various development periods, was high in purity (A260/A280 ratio ranged from 1.81 to 1.99 and A260/A230 ratio was over 2.0), and high in yield.
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