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Evaluation of in vitro gas production and nutrient digestibility of complete diets supplemented with different levels of thermotolerant yeast in Nellore rams

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Author(s): Ch. Harikrishna | M Mahender | Y Ramana Reddy | M Gnana Prakash | K Sudhakar | M Pavani

Journal: Veterinary World
ISSN 0972-8988

Volume: 5;
Issue: 8.000;
Start page: 477;
Date: 2012;
Original page

Keywords: Nellore rams | Thermotolerant yeast | In vitro gas production | Digestibility | N retention

ABSTRACT
Aim: The objective of the present study was to know the effect of dietary supplementation of varied levels of thermotolerant yeast to determine best levels for sheep diets by in vitro gas production. An in vivo study on Nellore rams was used for further evaluation of diets with three best levels of yeast (obtained from in vitro data) to determine diet with optimum yeast level for growing lambs by assessing nutrient digestibility, plane of nutrition and nitrogen balance. Materials and methods: A complete diet was formulated and supplemented with five levels (0 g/kg (D ); 1 g/kg (D ); 2 g/kg 1 2 (D ); 3 g/kg (D ); 4 g/kg (D ) and 5 g/kg (D ) of thermotolerant yeast (Saccharomyces cerevisiae, OBV-9) @ 5x108 cfu/g to 3 4 5 6 determine best levels for sheep diets by IVGP technique. An in vivo study was conducted on Nellore rams (39.75 0.24 kg body weight, aged 3 years) in a 4 x 4 latin square design for further evaluation of diets with three best yeast levels based on in vitro data, to determine optimum yeast level for diets of growing lambs by assessing nutrient digestibility, plane of nutrition and nitrogen balance. The rams were housed individually in metabolic cages that allowed separation of urine and faeces to evaluate digestibility of nutrients and N balance. Animals were given 10 days adaptation period followed by 7-day collection period, feed intake and refusals were recorded. During the digestibility and N balance study, feed, refusals and faeces were analyzed for dry matter (DM), organic matter (OM) and crude protein (CP) as per AOAC, USA, while fibre fractions like neutral detergent fibre (aNDF) and acid detergent fibre (ADF) were analyzed. Data were analyzed as per the procedures suggested by Snedecor, G. W. and Cochran, W. G. (1994) and the difference between treatment means was tested for significance by Duncan's multiple-range and F Test. Results: Higher (P
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