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The evolution of catalase and peroxidase activity in Phanerochaete chrysosporium grown on media containing beech and fir sawdust and under the influence of some amino acids

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Author(s): Alexandru Manoliu | Mihaela Balan | Lacramioara Oprica | Petronela Gradinaru

Journal: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
ISSN 1582-3571

Volume: 11;
Issue: 4;
Date: 2010;
Original page

ABSTRACT
The purpose of this paper is to highlight the influence of amino acids (histidine, glutamic acid, serine, valine, methionine, asparagine, α-alanine) on catalase and peroxidase activity in Phanerochaete chrysosporium grown on media containing fir and beech sawdust. In view of this research, Sabouraud medium carbon source was replaced by pine and beech sawdust 4 g per 100 ml medium and nitrogen source in the corresponding amino acid with different amounts of 100 mg N / L, resulting in the final eight work variants for each type of sawdust namely V1- valine, V2- histidine, V3- asparagine, V4- methionine, V5-glutamic acid, V6-α-alanine, V7- serine and V8 control who did not introduce any source nitrogen. Determination of catalase activity was done by spectrophotometric Sinha method and the peroxidase with ortho-dianisidine method at 11 days and 18 days after seeding, in fungus mycelium and culture liquid. Following the study found that the activity of these two enzymes was influenced by the type of amino acid, the nature of sawdust and fungus age.
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