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Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro

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Author(s): Milić Nenad S. | Gađanski-Omerović Gordana N. | Ašanin Ružica | Nisavić Jakov | Radojičić Marina

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 53;
Issue: 5-6;
Start page: 321;
Date: 2003;
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Keywords: PI3 virus | glycoprotein antigens | SDS-PAGE | BT cells | fusional test | hemolysis

ABSTRACT
The objective of our study was examination of the antigenic structure fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera.
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