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Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry

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Author(s): Soojin Park | Sunhee Park | Joo Yeon Oh | Sang Yun Han | Kyubong Jo | Han Bin Oh

Journal: Mass Spectrometry Letters
ISSN 2233-4203

Volume: 3;
Issue: 3;
Start page: 82;
Date: 2012;
Original page

Keywords: Zinc finger | Zinc ion | Electrospray ionization | Tartrate | Noncovalent complexes

ABSTRACT
The formation of zinc finger peptide−Zn2+ complexes in electrospray ionization mass spectrometry (ESI-MS) was examined using three different metal ion sources: ZnCl2, Zn(CH3COO)2, and Zn(OOC(CHOH)2COO). For the four zinc finger peptides (Sp1-1, Sp1-3, CF2II-4, and CF2II-6) that bind only a single Zn2+ in the native condition, electrospray of apo-zinc finger in solution containing ZnCl2 or Zn(CH3COO)2 resulted in the formation of zinc finger−Zn2+ complexes with multiple zinc ions. This result suggests the formation of nonspecific zinc finger−Zn2+ complexes. Zn(tartrate), Zn(OOC(CHOH)2COO),mainly produced specific zinc finger−Zn2+ complexes with a single zinc ion. This study clearly indicates that tartrate is an excellent counter ion in ESI-MS studies of zinc finger−Zn2+ complexes, which prevents the formation of nonspecific zinc finger−Zn2+ complexes.
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