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Expression of apoptotic proteins FasL, CPP-3, p53, and bcl-2 and their possible correlation with the progress of liver damage and biochemical disease activity in the course of chronic hepatitis type B

Author(s): Sylwia Serafińska | Krzysztof Simon | Agnieszka Hałoń

Journal: Polish Gastroenterology
ISSN 1232-9886

Volume: 16;
Issue: 4;
Start page: 271;
Date: 2009;
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Keywords: apoptosis | HBV | CHB

Introduction: Chronic hepatitis type B was diagnosed in all patients and other possible causes of hepatic diseases were excluded, i.e. alcoholism, toxic liver injury (occupational, drug induced), autoimmune liver diseases, metabolic liver diseases, other hepatotropic virusand HIVco-infection, tumor, and lung, kidney, and cardiovascular diseases. The possibility of coincidence of HBV infection and other significant factors affecting liver cell apoptosis was minimized during the enrollment process (e.g. enrolled patients took no drugs persistently in the past and during the trial). The aim of this study was to evaluate possible correlations between enhanced hepatocyte apoptosis (expressed by the presence of apoptotic bodies) and the degree of liver injury (in histopathological examination), biochemical activity of the disease, and HBeAg+/HBeAg- serologic pat-tern in a group of patients chronically infected with HBVwho had not undergone immunomodulating therapy with interferon a. Material and methods: Sixty-three individuals of Caucasian race and of both sexes (16 females, 25%, and 47 males, 75%) with an average age of 36.7±14.9 years were analyzed. Clinical evaluation, selected blood serum biochemical parameter analyses, serologic status determination, molecular studies, histopathological tests, immunohistochemical investigations of the apoptotic proteins p53, CPP-3, bcl-2, and FasL, and immu-nopathomorphological evaluation of the apoptotic proteins were conducted. The results were statistically analyzed. Results and conclusions: Enhanced hepatocyte apoptosis, evaluated by the expression of the selected proapoptotic proteins, in the patients with chronic HBV infection correlated with the degree of histopathological changes and biochemical activity of the disease. There was a relation-ship between the level of apoptosis and biochemical activity as well as histological liver damage in these patients.

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