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Expression of Estrogen Receptors α and β in Human Osteoblasts: Identification of Exon-2 Deletion Variant of Estrogen Receptor β in Postmenopausal Women

Author(s): Fang-Ping Chen | Todd Hsu | Chin-Hwa Hu | Wen-Der Wang | Kun-Chuang Wang | Li-Fen Teng

Journal: Chang Gung Medical Journal
ISSN 2072-0939

Volume: 27;
Issue: 02;
Start page: 107;
Date: 2004;
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Keywords: human osteoblast | postmenopausal women | ERα mRNA | ERβ mRNA | exon 2 deletion of ERβ mRNA.

Background: Postmenopausal osteoporosis is associated with estrogen deficiency.Estrogens have effects on bone metabolism, which are mediated by estrogenreceptors (ERs). If estrogen responsiveness is related to the ER expressionlevel, ER expression in postmenopausal women should be different from previousstudies using osteoblast lineage. We investigated the expression ofvariant isoforms of ER messenger ribonucleic acid (mRNA) in osteoblasts(OB) from postmenopausal women and a human osteosarcoma cell line, MG63.Methods: Osteoblast cultures were prepared from the upper femur of postmenopausalpatients or MG 63. For OB cultures at 5, 10, 15, 20, and 25 days, the expressionsof ERα and β mRNA were examined using reverse transcriptase-polymerasechain reaction.Results: In MG 63, ERβ mRNA was constantly and highly expressed during the25-day culture, whereas ERα mRNA was barely detected. In the primary OBcells, both ERα and β mRNA were transcribed during the 25-day culture, butexpression of ERα mRNA was much stronger than that of ERβ mRNA. Asplice variant form of ERβ mRNA that was missing the entire exon 2 (ERβ
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