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Expression of OCT4 in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis

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Author(s): Wei He | Ke Li | Feng Wang | Yan-Ru Qin | Qing-Xia Fan

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 18;
Issue: 7;
Start page: 712;
Date: 2012;
Original page

Keywords: Esophageal squamous cell carcinoma | Immunohistochemistry | OCT4 | Real-time polymerase chain reaction | Western blotting

ABSTRACT
AIM: To explore the expression pattern of OCT4 in human esophageal squamous cell carcinoma and its significance in diagnosis and prognosis. METHODS: Using real-time polymerase chain reaction (PCR), Western blotting, immunocytochemistry and immunohistochemistry, the expression of OCT4 in three esophageal squamous cancer cell lines, KYSE70, KYSE140 and KYSE450, was characterized. OCT4 expression was investigated in a series of 153 esophageal squamous cell carcinoma samples using immunohistochemistry and explored its association with clinicopathological features. RESULTS: Immunohistochemically, OCT4 positive immunostaining was observed in cancer cell nuclei. OCT4 was variably expressed in three esophageal squamous cancer cell lines. Among 153 specimens, 105 (68.7%) were negative or weakly positive for OCT4 staining; 21 (13.7%) were moderately positive and 27 (17.6%) were strongly positive. Higher expression level of OCT4 was significantly associated with higher histological grade (P < 0.001) and poor clinic outcome (P < 0.001). CONCLUSION: The expression of OCT4 enables the tumor to have a higher degree of stemness, which in turn results in a poorer clinical outcome for patients with esophageal squamous cell carcinoma.
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