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Expression of pattern recognition receptors in liver biopsy specimens of children chronically infected with HBV and HCV

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Author(s): Iwona Mozer-Lisewska | Arleta Kowala-Piaskowska | Anna Mania | Renata Jenek | Husam Samara | Elżbieta Kaczmarek | Jan Sikora | Wojciech Służewski | Jan Żeromski

Journal: Folia Histochemica et Cytobiologica
ISSN 0239-8508

Volume: 49;
Issue: 3;
Start page: 410;
Date: 2011;
Original page

Keywords: pattern recognition receptors | viral hepatitis | liver biopsy | children | immunohistochemistry

ABSTRACT
Pattern recognition receptors (PRRs) constitute a pivotal arm of innate immunity. Their distributionis widespread and not limited to cells of the immune system. Following our previous findings concerning theexpression of Toll-like receptors (TLRs) 2, 3 and 4 in chronic viral hepatitis C of children, we wished to search forother PRRs, including other TLRs, NOD-like receptors (NLRs) and RIG-1-like helicase receptors (RLR) ininfected hepatocytes. Liver biopsy fragments from ten children with chronic hepatitis B and C were used and twoothers in which hepatotropic virus infection was excluded. Frozen sections of liver samples were subjected to ABCimmunohistochemistry (IHC) following incubation with a set of antibodies. Results of IHC findings were screenedfor correlation with clinical/laboratory data of patients. It was found that several PRRs could be shown in affectedhepatocytes, but the incidence was higher in hepatitis C than in B. In hepatitis C, TLR1, 2, 4, NALP and RIG-1helicase showed the most marked expression. In hepatitis B, TLR1, 3, 9, NOD1 and NALP expression were themost conspicuous. Expression PRRs in liver from hepatitis of unknown origin was much lower. It was also the casein cytospins from human hepatoma cell line. Several correlations between PRRs expression and clinical findings inpatients could be shown by statistical exploration. In conclusion, this data suggests some role for PRRs in thepathogenesis of chronic viral hepatitis. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 410–416)
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