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Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation

Author(s): Nugent Rebecca | Johnsson Anna | Fleharty Brian | Gogol Madelaine | Xue-Franzén Yongtao | Seidel Chris | Wright Anthony | Forsburg Susan

Journal: BMC Genomics
ISSN 1471-2164

Volume: 11;
Issue: 1;
Start page: 59;
Date: 2010;
Original page

Abstract Background Histone acetyltransferase enzymes (HATs) are implicated in regulation of transcription. HATs from different families may overlap in target and substrate specificity. Results We isolated the elp3+ gene encoding the histone acetyltransferase subunit of the Elongator complex in fission yeast and characterized the phenotype of an Δelp3 mutant. We examined genetic interactions between Δelp3 and two other HAT mutants, Δmst2 and Δgcn5 and used whole genome microarray analysis to analyze their effects on gene expression. Conclusions Comparison of phenotypes and expression profiles in single, double and triple mutants indicate that these HAT enzymes have overlapping functions. Consistent with this, overlapping specificity in histone H3 acetylation is observed. However, there is no evidence for overlap with another HAT enzyme, encoded by the essential mst1+ gene.
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