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Extended Immunization of Rats using Microencapsulated Cobra Venom

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Author(s): H.F. Salem | Aly Fahmy | Ahmed M.A. Ali

Journal: British Journal of Pharmacology and Toxicology
ISSN 2044-2459

Volume: 2;
Issue: 1;
Start page: 43;
Date: 2011;
Original page

Keywords: BSA | Copra venom | ELISA | microspheres | sustained release

ABSTRACT
Production of a vaccine with an extended release kinetic and with a high immunization response has an economic value in the third world countries. In this study, production and evaluation of bovine serum albumin microspheres that are incorporating cobra venom were carried out. The microcapsules were prepared using modified emulsification-polymerization technique. They were evaluated for their size using photon correlation spectroscopy and for their morphology using scanning electron microscopy. The release kinetics of the venom from the chosen formula was following Higushi model with r2 >0.97. The average microsphere size of the best formula was 5.6 :m and the encapsulation efficiency approached 82%. The pharmacokinetic parameters of the microencapsulated cobra venom showed a significant difference from the free venom formulations in terms o f prolonged stimulation of in-vivo immune response (production of antibodies). The method of preparation and evaluation of microspheres were characterized by being easy to perform, yielding high entrapment efficiency and maximum induction of immune response, which enables availability of a safe, effective and economic vaccine.
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