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Extraction of Algae Oil from Nannocloropsis sp.: A Study of Soxhlet and Ultrasonic-Assisted Extractions

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Author(s): B. Wiyarno | R.M. Yunus | M. Mel

Journal: Journal of Applied Sciences
ISSN 1812-5654

Volume: 11;
Issue: 21;
Start page: 3607;
Date: 2011;
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Keywords: free fatty acid (FFA) | saponification number | Microalgae oil | ultrasonic assisted extraction (UAE) | soxhlet extraction (SE)

ABSTRACT
This study aims at describing the characteristics of the microalgae oil extraction from Nannochloropsis sp. using soxhlet and ultrasonic. There were two extraction processes that were investigated, Soxhlet-assisted Extraction (SE) and Ultrasonic-assisted Extraction (UE) and both used ethanol solvent. A combination of several criteria was selected to find the characteristics of each extraction. In the SE, a variety of ethanol concentration and time were used; whereas in the UE, a variety of ethanol volume, time and temperature were applied. The given frequency for all UE treatments was 40 kHz. The quality of algae oil proceeded by SE was shown by the level of FFA (Free Fatty Acid) and saponification number. In the SE study, the best combination was gained when the ethanol concentration was 70% and the given time was 200 min in which the FFA level was 9.4% and the saponification number was 286.8. While in the UE study, 51.6 min, 98% of ethanol concentration and 69.62C were the best circumstance in which the quantity of the oil yield got its maximum. In SE, the higher solvent concentration, the higher FFA level and saponification number were gained. However, after reaching the peak at particular circumstance, the saponification number decreased gradually. Meanwhile, UE reduced the length of extraction time.

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