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Extraction and optimization of molecular marker compounds from Asparagus racemosus using HPLC with ELS detector

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Author(s): Somasekhar Penumajji1, Varaprasad Bobbarala2* and K. Chandrasekhar Naidu2

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 3;
Issue: 1;
Start page: 159;
Date: 2010;
Original page

Keywords: Asparagus racemosus | Evaporative light scattering detector. High Performance Liquid chromatography

ABSTRACT
Asparagus racemosus is known as Shatavari in Sanskrit. Dried roots of A. racemosus were extracted with organic solvents in different ratios and were analyzed using high performance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD). The markers of our interest in A. racemosus are Shatavari I and Shatavari IV. Our research reports suggested that High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) is used as an alternative and effective tool for the identification of the compounds which do not contain strong chromophores. Present study concludes that 57.3 % ethyl acetate in methanol is the optimum solvent composition from which we can get the maximum recovery of Shatavarin I and Shatavarin IV markers which was efficiently determined using HPLC with ELSD.
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