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A fluorescent-labeled microcystin-LR terbium cryptate

Author(s): Oliveira Eduardo J. A. | Vila Nova Suzana P. | Alves-Jr Severino | Santa-Cruz Petrus | Molica Renato J. R. | Teixeira Antonio | Malageño Elizabete | Lima Filho José L.

Journal: Journal of the Brazilian Chemical Society
ISSN 0103-5053

Volume: 17;
Issue: 2;
Start page: 243;
Date: 2006;
Original page

Keywords: terbium cryptate | microcystin-LR | cyanobacteria

A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well.
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