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Fractionation of antibacterial extracts of Syzygium cumini (Myrtaceae) seeds

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Author(s): Vijay Kothari | Sriram Seshadri | Priti Mehta

Journal: Research in Biotechnology
ISSN 2229-791X

Volume: 2;
Issue: 6;
Start page: 53;
Date: 2011;
Original page

Keywords: Bacteriostatic | Microwave assisted extraction (MAE) | MIC | TLC | HPLC

ABSTRACT
Antibacterial activity of Syzygium cumini seed extracts prepared in methanol and ethanol was evaluated by disc diffusion and broth dilution assays. Both extracts exerted a broad spectrum of bacteriostatic action against different gram-positive and gram-negative bacteria. Their minimum inhibitory concentration (MIC) against susceptible organisms ranged from 154-656 μg/mL. Highest total activity was registered by the ethanol extract against Staphylococcus epidermidis. Extracts were separated on TLC (thin-layer chromatography) plates, and separated components were individually tested for their antibacterial activity. HPLC (high performance liquid chromatography) analysis indicated presence of gallic acid and quercetin in the methanolic extract.
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