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Frequency and Antibiotic Susceptibility Pattern of Amp C Beta Lactamase Producing Bacteria Isolated from a Tertiary Care Hospital of Pakistan

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Author(s): Afreenish Hassan | Javaid Usman | Fatima Kaleem | Maria Omair | Ali Khalid | Muhammad Iqabal

Journal: International Journal of Collaborative Research on Internal Medicine & Public Health
ISSN 1840-4529

Volume: 3;
Issue: 3;
Start page: 219;
Date: 2011;
Original page

Keywords: Amp C beta lactamases | Antimicrobial resistance | three dimensional extract test

ABSTRACT
Objective: Amp C beta lactamases are cephalosporinases which hydrolyze cephamycins and are poorlyinhibited by clavulanic acid. Amp C beta lactamases confer resistance to a wide variety of antibiotics andpose both diagnostic and therapeutic challenges, The objective was to detect the frequency and antibioticsusceptibility pattern of Amp C beta lactamase producing bacteria isolated from a tertiary care hospital ofPakistan.Methodology: Organisms were isolated from various clinical specimens. First, the screening of theisolates was done by using cefoxitin disc. Screen positive organisms were subjected to three dimensionalextract test for detection of Amp C beta lactamases.Results: From a total of 100 organism tested, 64 organisms were positive on cefoxitin screen test. Out ofthese 64, 40 (62.5%) showed the presence of Amp C beta lactamase. (E.coli 18, K.pneumoniae 14,K.oxytoca 1, Enterobacter species 5, Citrobacter freundii 2) by three dimensional extract test. Theantibiotics found out to show good activity against these resistant bacteria include meropenem andtigecycline. This is the first study to determine the frequency of Amp C beta lactamases from Pakistan.Conclusion: This study shows a high frequency of Amp C beta lactamase producing isolates from ahospital, which may lead to serious therapeutic problems.
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