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FTIR analysis of polyhydroxyalkanoates by a locally isolated novel Bacillus sp. AS 3-2 from soil of Kadi region, North Gujarat, India

Author(s): Kamlesh Shah

Journal: Journal of Biochemical Technology
ISSN 0974-2328

Volume: 3;
Issue: 4;
Start page: 380;
Date: 2012;
Original page

Keywords: Bacillus sp. Biopolymer | PHA | FTIR spectroscopy | Chloroform | Sudan black B

Soil sample collected from industrial area of Kadi and Mehshana, India. Twelve bacterial isolates were found to be the most promising PHA accumulating bacteria among 15 isolates screened from soil. Screening for PHA was done by Sudan black staining. PHA extraction was carried out by chloroform digestion method. Biochemical and 16s rRNA analysis showed that PHA producing bacteria belong to Bacillus genera with Maximum production of PHA was analyzed by U.V spectrophotometer and finally it was characterized by FTIR spectroscopy.
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