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Functional characterisation of semen in honeybee queen (A.m.ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination

Author(s): Marco Lodesani | Donatella Balduzzi | Andrea Galli

Journal: Italian Journal of Animal Science
ISSN 1594-4077

Volume: 3;
Issue: 4;
Start page: 385;
Date: 2010;
Original page

Keywords: Apis mellifera ligustica | Queen honey bee | Spermatheca | Spermatozoa | Instrumental insemination

Differences over time in the quality of semen present in the honey bee (Apis mellifera ligustica) queen spermatheca werestudied. An increase in the non-vital spermatozoa was shown to be evident (P>0.05) between the 12th and 24th month.The study of semen viability demonstrated that the passage of the semen to the spermatheca is due to sperm motility.In the queen inseminated with non-viable spermatozoa, no semen was detected in the spermatheca. Queens inseminatedtwice with a Hyes solution/semen mixture (1:1) stored as many spermatozoa in their spermatheca as those inseminatedonce with the classic technique. Queen replacement, oviposition and other functional characteristics were similarto those observed in the classic insemination procedure.
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