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Genetic Differentiation of Crucus Species by Random Amplified Polymorphic DNA

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Author(s): AH Beiki

Journal: Genetic Engineering and Biotechnology Journal
ISSN 2150-3516

Volume: 2010;
Start page: GEBJ-18;
Date: 2010;
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Keywords: Genetic differentiation | Crucus | RAPD

ABSTRACT
Saffron has for decades been the world's most expensive spice by weight. It is a spice derived from the flower of the saffron (Crocus sativus), a species of Crocus in the family Iridaceae. Considering the importance of germplasm characterization and evaluation particularly in saffron, the present study was formulated with an objective to access the structure of genetic diversity among the accession of saffron at molecular levels. Twenty four wild type and six cultivated colonies were gathered from seven different states and were evaluated by 26 random amplified polymorphic DNA (RAPD) molecular markers that we were expecting, to assess degree of genetic variability between them. A total of 322 bands were scored corresponding to an average of 16.6 bands per primer with 281 bands showing polymorphism (87.3%) and values of polymorphism information content (PIC) varying from 0.24 to 0.72. Results obtained after use of RAPD markers showed that “Operon M10” is more accurate and its application is recommended. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters. Cluster I comprised four cancellatuse species. Cluster-II comprises of 26 cultivars that were further differentiated into two sub-clusters. All genotypes in subgroup IIa include six cultivated ones. The PCoA revealed a pattern in which the individuals were assigned into five separate groups. Cluster analysis and 2D and 3D principal component analysis demonstrated the effectiveness of RAPD markers in grouping of accessions under study, and analysis of their genetic relationships.
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