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Genetic diversity among Indian phytopathogenic isolates of Fusarium semitectum Berkeley and Ravenel

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Author(s): Avinash Ingle | Mahendra Rai

Journal: Advances in Bioscience and Biotechnology
ISSN 2156-8456

Volume: 02;
Issue: 03;
Start page: 142;
Date: 2011;
Original page

Keywords: Fusarium | Genetic Diversity | RAPD | Macroconidia | UPGMA

ABSTRACT
We report total ten isolates of F. semitectum recovered from different hosts. Identity of these isolates was determined by morphological and cultural characteristics and confirmed by RAPD-PCR analysis using forty random primers. Morphologically all the ten isolates showed similarity, but based on RAPD-PCR analysis, these isolates can be categorized in three groups depending upon similarity co-efficient. Genetic similarity coefficients between pair wise isolates varied from 0.00 to 1.95 based on an unweighted paired group method of arithmetic average (UPGMA) cluster analysis. RAPD-PCR technique can be used as an important tool for the genetic differentiation among isolates of F. semitectum.
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