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Genetic Site Determination of Antibiotic Resistance Genes in Pseudomonas aeruginosa by Genetic Transformation

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Author(s): K.K. Adel | S.S. Sabiha

Journal: British Journal of Pharmacology and Toxicology
ISSN 2044-2459

Volume: 1;
Issue: 2;
Start page: 85;
Date: 2010;
Original page

Keywords: Antibiotics | chromosomal DNA | multi-drug resistance | transformation | plasmid | Pseudomonas aeruginosa

ABSTRACT
To determine the site of antibiotic resistant genes in Pseudomonas aeruginosa, 50 P. aeruginosa were isolated out of 250 samples from hospitalized patients in different hospitals in Sulaymani city, Iraq, more over isolated from soil dustbin and sewage water. The isolates were identified according to the cultural, morphological and biochemical tests, in addition to API 20E system. The isolated bacteria were screened for their resistance to seventeen commonly used antibiotics. The isolated P. aeruginosa show different pattern of antibiotic resistance, they grouped in to 34 antibiotype. Organisms were 100% resist to (Amp, Cef, Er, Gm, Lin, Pip and Cln), the lowest resistant recorded was 35% for Rif. Six P. aeruginosa isolates 12% revealed resistant to all seventeen used antibiotics, while nine isolates 18% were sensitive to one antibiotic, and 10 isolates were sensitive to two antibiotics, and remaining 50 isolates were sensitive to 3-5 antibiotics. Transformation process appeared that the genes responsible for all tested antibiotics except Lin in P1 isolate, and Lin and Tob in P8 isolate are located on plasmid DNA in isolated P. aeruginosa.
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