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Genome-wide mapping of imprinted differentially methylated regions by DNA methylation profiling of human placentas from triploidies

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Author(s): Yuen Ryan KC | Jiang Ruby | PeƱaherrera Maria S | McFadden Deborah E | Robinson Wendy P

Journal: Epigenetics & Chromatin
ISSN 1756-8935

Volume: 4;
Issue: 1;
Start page: 10;
Date: 2011;
Original page

ABSTRACT
Abstract Background Genomic imprinting is an important epigenetic process involved in regulating placental and foetal growth. Imprinted genes are typically associated with differentially methylated regions (DMRs) whereby one of the two alleles is DNA methylated depending on the parent of origin. Identifying imprinted DMRs in humans is complicated by species- and tissue-specific differences in imprinting status and the presence of multiple regulatory regions associated with a particular gene, only some of which may be imprinted. In this study, we have taken advantage of the unbalanced parental genomic constitutions in triploidies to further characterize human DMRs associated with known imprinted genes and identify novel imprinted DMRs. Results By comparing the promoter methylation status of over 14,000 genes in human placentas from ten diandries (extra paternal haploid set) and ten digynies (extra maternal haploid set) and using 6 complete hydatidiform moles (paternal origin) and ten chromosomally normal placentas for comparison, we identified 62 genes with apparently imprinted DMRs (false discovery rate

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