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GENOMIC SURVEY, CHARACTERIZATION AND EXPRESSION PROFILE ANALYSIS OF THE YELLOW STRIP LIKE GENE FAMILY IN RICE AND ARABIDOPSIS

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Author(s): RAKESH MENNA, MAHIMA DUBEY AND GIRISH CHANDEL

Journal: International Journal of Biotechnology Applications
ISSN 0975-2943

Volume: 3;
Issue: 2;
Start page: 55;
Date: 2011;
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Keywords: Digital expression analysis | Metal transporter | Phytosiderophore | YSL- gene

ABSTRACT
Genes in the YSL gene family encode for the metal transporter in plants. In this study, a comprehensive andcomparative computational analysis of identified 18 and 8 YSL family genes in rice and Arabidopsis respectively have beencarried out presenting a complete overview on gene structures, phylogeny, conserved motifs their distribution andrelatedness, intron/ exon distribution and expression. The analysis in all indicated that YSL genes are relatively conservedgene family showing similarity on the basis of evolution and indicates their common ancestral origin. The multi-sequencealignment showed primary and secondary level of conservation at sequence level of YSLs in both rice Arabidopsis. YSLgene intron/ exon structure and domains were also found to be conserved during the evolution. Characterization of YSLs bysequence tag based methods of expression profiling viz ESTs and MPPS signatures showed a total of 833 ESTs and 382MPSS tags co-localizing with the OsYSLs and 269 ESTs and 219 MPSS tags with AtYSLs. The expression pattern furtheranalyzed digitally, by in silico microarrays in 22 tissues libraries, 8 stages of development and under different stimulirevealed assorted and preferential patterns of expression and provide an evidence for spatiotemporal regulation of thesegenes throughout plant development. OsYSL6 gene of rice showed very high as well as tissue specific expression underiron and phosphorous interaction by digital microarrays which were equally supported by ESTs and MPSS abundance.Similarly in Arabidopsis AtYSL1, 3 and 6 showed prominent expression in senescent leaves indicating their role inremobilization of metals.
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