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Genotoxic assessment of the Carapa guianensis Aublet seed oleaginous extract in Balb/c mice micronucleus assay

Author(s): Daniel Francisco Arencibia Arrebola | Luis Alfredo Rosario Fernández | Livan Delgado Roche | Adriana Alonso Laurencio | Yolanda Emilia Suárez Fernández, | Alexis Vidal Novoa

Journal: RETEL : Revista de Toxicología en Línea
ISSN 1668-091X

Volume: 39;
Start page: 1;
Date: 2012;
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Keywords: Oleaginous extract | Carapa guianensis | micronucleus | Balb/c mice

The seed oil extract of Carapa guianensis has various biomedical applications. Recently was evaluated expressing great potential as in vivo antioxidant assays. The aimed of this study was to evaluate the genotoxic potential of the seed oil Carapa guianensis extract in the micronucleus assay of bone marrow cells in Balb/c mice. We were formed 5 experimental groups. A placebo group (Tween 65, 2%), three dose levels of the extract (400, 1 000 and 2 000 mg/kg) administered orally for 14 days. Finally a positive control group treated with cyclophosphamide (CF), at a dose of 50 mg/kg intraperitoneally 48 and 24 h before euthanasia. Were administered 5 animals/sex/group. After 14 days of dosing the animals were euthanized by cervical dislocation and the bone marrow of femur was extracted to proceed with the micronucleus cytogenetic technique. The results between controls and treated with the extract did not differ for the two sexes in the cytotoxicity index (PE/NE) and the PE percent with micronucleus as genotoxicity index. However the controls and treated with the extract were differed from the group treated with CF, validating our results. We conclude that the oleaginous extract of the Carapa guianensis seeds has not genotoxic potential in the formation of micronucleus in Balb/c mice bone marrow of both sexes.
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