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Genotoxicity of Chlorpyrifos and the Antimutagenic Role of Lettuce Leaves in Male Mice

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Author(s): Kamilia Badrakhan Abdelaziz | Aida Ibrahim El Makawy | Ali Zain El-Abidin Abd Elsalam | Ahmed Mohamed Darwish

Journal: Comunicata Scientiae
ISSN 2176-9079

Volume: 1;
Issue: 2;
Start page: 137;
Date: 2010;
Original page

Keywords: organophosphate | chromosome aberration | somatic cells | germ cells | sperm analysis

ABSTRACT
Chlorpyrifos [O O-diethyl-O-(3 5 6-trichloro-2-pyridyl)-phosphorothioate] is one of the most widelyused organophosphate insecticides. Previous studies proved that chlorpyrifos, at different doses,induced genotoxicity. In Egyptian foods, the residual levels of pesticides are often higher than thosefound in developed country ones. So the aim of this research was to evaluate the genotoxicity of theinsecticide chlorpyrifos at doses equal to its maximum residue limit (MRL) in the leafy vegetables, itsdouble and quadruple (0.5, 1 and 2 mg/kg body weight) in somatic and germ cells of male mice.In addition to that, evaluating the role of lettuce leaves as antigenotoxic in reducing the genotoxiceffects of chlorpyrifos tested doses when concurrently administrated to these animals. The studywas conducted on adult male laboratory mice at three levels: bone marrow cells as a model formitotic chromosome aberrations, spermatocytes as a model for meiotic chromosomes and spermcount and morphology. The results of the present study indicate that the treatment of male micewith chlorpyrifos by oral gavages for three months induced significant increase in the frequencies oftotal chromosomal aberrations in both somatic and germ cells in relation to control groups. Resultsof the sperm analysis showed that chlorpyrifos induced significant decrease in the sperm countwhen compared to negative control. Furthermore, it induced significant increase in head and tailsperm abnormalities, among which coiled tail was considered the most obvious sperm abnormalityinduced by chlorpyrifos. At the same time, the present study indicated that lettuce leaves feedconcurrently with three doses of chlorpyrifos could not protect cells from damage.
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