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GREEN SYNTHESIS OF GOLD NANOPARTICLES USING TOONA CILIATA METHANOL BARK EXTRACT AND THEIR CHARACTERISATION

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Author(s): Kaushik Rajan | Mann Avninder Singh | Sandhu Bharpur Singh | Parihar Vidya Bhushan Singh

Journal: International Research Journal of Pharmacy
ISSN 2230-8407

Volume: 3;
Issue: 9;
Start page: 115;
Date: 2012;
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Keywords: Toona ciliata | Methanol Bark Extract | Green Synthesis | Gold Nanoparticles | Transmission Electron Microscopy.

ABSTRACT
In the present study, we identified and justified the use of MeOH extract from Toona ciliata bark as a reducing and capping agent for the ecofriendly synthesis of gold nanoparticles on the basis of modern analytical techniques. The reduction of 1.0 mM aqueous solution of aurochloric acid with 1 ml, 1% w/v aqueous solution of MeOH bark extract from Toona ciliata has resulted in the formation of stabilised Gold Nanoparticles (AuNPs). The synthesised gold particles showed a surface plasmon band around 550 nm when analysed via UV-Visible Spectroscopy, indicated the gold particles of nano dimensions (10-9 m). The Transmission Electron Microscopy (TEM) study of gold nano particles revealed the formation of spherical, poly dispersed nanoparticles of varying sizes ranging from 40-75 nm along with encapsulating cage. The time for the synthesis of gold nano particles was noted to be 30 minute. The preliminary phytochemical analysis of methanol extract form the bark confirmed the presence of alkaloids, glycosides, flavanoids, tannins and reducing sugars. The results of the present study clearly reveal the Toona ciliata methanolic bark extract as a new, novel and renewable, cost effective, reducing and capping agent for the application in the field of nanobiotechnology as well as pharmaceutical sciences. Further, the ecofriendly approach developed for AuNPs synthesis with Toona ciliata MeOH bark extract is the rapid and cost effective alternative to the traditional chemical methods of AuNPs synthesis.
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